关键词: 卵巢癌, 柚皮苷, 增殖, 侵袭

Abstract: ObjectiveTo investigate the effect of naringin on proliferation， apoptosis and migration on ovarian cancer cell line SKOV3. MethodsNaringin of 1， 5， 10， 20 μmol／L was used to treat SKOV3 cells at logarithmic growth phase. SKOV3 cells without naringin treatment were used as control. The proliferation of cells treated with naringin at different concentrations at 48 h and 20 μmol／L naringin for 12， 24， 36 and 48 h were detected by MTT assay. SKOV3 cells treated with different concentrations of naringin for 48 h were collected. AnnexinVFITC／PI double staining and PI single staining flow cytometry were used to detect the apoptosis and cell cycle of SKOV3 cells， respectively. Transwell method was used to evaluate the migration ability by detecting the number of cell penetrating membrane. The relative levels of pAkt and PTEN were detected by Western blotting. 
ResultsMTT detection showed that following treatment with naringin at 1， 5， 10， 20 μmol／L for 48 h， the proliferative rate of SKOV3 cells decreased. In addition to 1 μmol／L， the proliferative rates of SKOV3 cells treated with 520 μmol／L were lower than control group （P＜0.05）. The proliferative rates were （61.95±6.87）％ and （50.58±6.09）％ of 10 and 20 μmol／L at 48 h， and there was no significant difference （P＞0.05）. The proliferative rates were （83.93±5.54）％， （73.10±3.58）％， （61.95±5.01）％ and （53.00±7.67）％ of 20 μmol／L at 12， 24， 36 and 48 h， were lower than those of control group （P＜0.05）. Compared with control group， the proportion of cells at G0／G1 phase and the apoptotic rate increased， while the proportion of cells at S phase decreased in cells exposure to 120 μmol／L naringin at 48 h （P＜0.05）.Following treatment with 1， 5， 10 and 20 μmol／L naringin， the number of cell penetrating membrane was 61.83±6.77， 47.17±5.35， 32.17±5.95 and 2083±306， the relative levels of pAkt were 0545+0050， 0.373+0.035， 0.280+0.054 and 0.173+0.034， and the relative levels of PTEN were 0.357+0.054， 0.468+0.085， 0602+0.063 and 0.728+0.03， better than those of control group （P＜0.05）. 
ConclusionNaringin can significantly inhibit the proliferation of ovarian cancer cell line SKOV3， promote its apoptosis， and reduce its invasive ability， which may be related to the inhibition of PTEN／Akt signaling pathway activation. It provides a possible clue for the clinical treatment of ovarian cancer.

Key words: Ovarian cancer, Naringin, Proliferation, Invasion