肝细胞癌组织中HMGA2表达及其临床意义

摘要/Abstract

摘要： 目的探讨肝细胞癌（HCC）组织中高迁移率族蛋白A2（HMGA2）的表达及其与临床病理特征和预后的关系。
方法收集本院2011年1月至2012年12月经手术切除的120例HCC组织、癌旁组织和正常肝组织，采用免疫组化SP法检测以上组织中的HMGA2表达情况，分析HMGA2表达与HCC临床病理特征（性别、年龄、分化程度、TNM分期、肝门淋巴结转移、癌灶数量、肿瘤大小和肝内转移）的关系，根据随访资料分析不同HMGA2表达HCC患者的预后情况。
结果HMGA2阳性表达在细胞核中呈棕黄色或棕褐色。HCC组织中HMGA2阳性率为6417％（77／120），高于癌旁组织的834％（10／120）和正常组织的417％（5／120），差异有统计学意义（P＜005）；癌旁组织和正常组织HMGA2阳性率的差异无统计学意义（P＞005）。HMGA2表达与HCC分化程度、TNM分期、肝门淋巴结转移、癌灶数量、肿瘤大小和肝内转移有关（P＜005），与性别和年龄均无关（P＞005）。HMGA2阳性者的中位总生存期（OS）为135个月，阴性者的中位OS＞600个月，HMGA2阴性者的中位OS长于阳性者（P＜005）；HMGA2表达、TNM分期、肝内转移、肝门淋巴结转移、癌灶数量、肿瘤大小和分化程度与HCC患者的OS有关（P＜005）。经Cox回归模型进行多因素分析显示HMGA2阳性表达是影响HCC预后的独立危险因素（HR=2516，95％CI：1643～3855）。
结论HCC组织内HMGA2蛋白表达升高，且参与HCC的发生发展，HMGA2阳性表达者的预后较差，该蛋白可能作为一个评估HCC预后的潜在指标。

关键词: 肝细胞癌, 高迁移率族蛋白A2, 临床意义, 预后

Abstract: ObjectiveTo investigate the expression of high mobility group box A2 （HMGA2） in hepatocellular carcinoma （HCC） tissues as well as its relationship with clinicopathological parameters and prognosis.
MethodsIn this study， 120 cases of surgically resected HCC tissues， paracancerous tissues and normal liver tissues were collected from January 2011 to December 2012. The expression of HMGA2 in the above tissues was detected by immunohistochemical SP. The expression of HMGA2 and HCC clinicopathological parameters （sex， age， differentiation， TNM staging， hepatic hilar lymph node metastasis， number of tumor foci， tumor size and intrahepatic metastasis） were analyzed. The prognosis of HCC patients with different HMGA2 expression was analyzed according to the followup data.
ResultsImmunohistochemistry showed that the nuclei with HMGA2 expression in cells were brownish yellow or tan. The positive rate of HMGA2 in HCC tissue was 6417％（77／120）， higher than 834％（10／120） of paracancerous tissues and 417％（5／120） of normal tissue （P＜005）. There was no significant difference in the positive rates of HMGA2 between paracancerous tissues and normal tissues （P＞005）. The expression of HMGA2 was related to the degree of differentiation of HCC， TNM staging， hepatic hilar lymph node metastasis， number of tumor foci， tumor size and intrahepatic metastasis （P＜005）， but not related to sex and age （P＞005）. The median overall survival （OS） were 135 months for patients with HMGA2 positive expression and ＞600 months for patients with HMGA2 negative expression. Patients with HMGA2 negative expression had a longer median OS （P＜005）. The results of single factor analysis showed that HMGA2 expression， TNM staging， intrahepatic metastasis， hilar lymph node metastasis， number of tumor foci， tumor size and degree of differentiation were related to OS of HCC patients. Multivariate analysis of Cox regression showed that HMGA2 expression was an independent risk factor for HCC prognosis （HR=2516， 95％CI： 16433855）.
ConclusionThe expression of HMGA2 protein in HCC tissue is elevated， and it participates in the development of HCC. The prognosis of HMGA2 positive expression is poor. This protein may be a potential prognostic indicator of HCC.

Key words: Hepatocellular carcinoma, High mobility group protein A2, Clinical significance, Prognosis

刘湘冬, 谢卫民, 李伟. 肝细胞癌组织中HMGA2表达及其临床意义[J]. 临床肿瘤学杂志, 2018, 23(8): 706-710.

LIU Xiangdong, XIE Weimin, LI Wei. . Expression analysis of HMGA2 in hepatocellular carcinoma and its clinical significance[J]. Chinese Clinical Oncology, 2018, 23(8): 706-710.

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