Abstract: Objective To identify the differentially expressed genes in residual cervical cancer after radiotherapy by cDNA microarray.
Methods Cervical cancer tissues from 3 patients before radiation and after 50 Gy dose of radiation were tested by cDNA microarray to select differentially expressed genes. The microarray results were validated by quantitative real-time PCR. Results Hierarchic cluster analysis identified that 238 differentiated genes exhibited ≥3.0fold change，among which 111 genes were found to be in persistent up-regulation and 127 in persistent down-regulation after 50 Gy dose of radiation. These genes were involved in cell apoptosis and antophagy, cell energy metabolism, angiogenesis，cell apoptosis，cell cycle regulation，cell signaling，DNA damage repair, cell adhesion，and etc. The expression of CXCL12，CD74，FGF7，COL14A1，CD44 and ATM genes was up-regulated after radiation（P＜0.05）. Meanwhile,PRC1 and RAD54L were downregulated after radiation（P＜0.05）. Conclusion The differentially expressed genes identified by cDNA microarray are hoped to provide a foundation for the development of new diagnostic strategies and treatment for radiation resistance in cervical cancer.