关键词: 食管鳞癌, 长链非编码 RNA, ENST00000589379, 细胞迁移

Abstract: ObjectiveTo investigate the level of long noncoding RNA（lncRNA） ENST00000589379 in tissues and its influence on cell migration of esophageal squamous cell carcinoma（ESCC）. 
MethodsSixtytwo cases of ESCC and paired paracancerous tissues from January 2014 to December 2014 were collected. Using realtime quantitative PCR（QPCR）， the level of ENST00000589379 in the above tissues was investigated. The ENST00000589379 levels in ESCC tissues and adjacent normal tissues were compared. The relationship between ENST00000589379 levels and clinicopathological parameters（age， sex， tumor size， differentiation， T stage， lymph node metastasis and TNM stage） of ESCC was analyzed. By silencing and overexpressing ENST00000589379 in ESCC cells TE1 and Eca109， the relationship between the expression level and metastasis of ESCC cells was evaluated. 
ResultsQPCR results showed that the levels of ENST00000589379 in ESCC tissues and cells were higher than those in adjacent tissues and normal esophageal mucosal epithelial cells（P＜005）. The levels of ENST00000589379 in ESCC were not related to age， sex， tumor size， differentiation， and T staging， but related to lymph node metastasis and TNM staging（P＜005）. The ENST00000589379 level of tissues with lymph node metastases was 559±127， higher than 420±156 of those without lymph node metastases， and the level of ENST00000589379 in tissues of TNM ⅢⅣ stage was 587±107， higher than 423±154 of ⅠⅡ stage（P＜005）. Silencing ENST00000589379 could obviously reduce the metastasis of TE1 and Eca109 cell lines while overpressing ENST00000589379 could promote the metastasis of the two cell lines. 
ConclusionThe increased level of ENST00000589379 in ESCC， especially in lymph node metastasis and late TNM stage， has a significant effect on the migration of ESCC， and is of certain significance in the prevention and treatment of ESCC.

Key words: Esophagus squamous cell carcinoma, Long noncoding RNA, ENST00000589379, Cell migration