乳腺癌组织中lncRNA CCHE1水平及其临床意义

临床肿瘤学杂志 ›› 2018, Vol. 23 ›› Issue (6): 519-523.

乳腺癌组织中lncRNA CCHE1水平及其临床意义

武汉华中科技大学同济医学院附属同济医院病理研究所

出版日期:2018-06-30 发布日期:2018-08-29

Expression of lncRNA CCHE1 and its clinical significance in breast cancer tissues

Institute of Pathology， Tongji Hospital， Tongji Medical College， Huazhong University of Science and Technology

Online:2018-06-30 Published:2018-08-29

摘要/Abstract

摘要： 目的探讨长链非编码RNA（lncRNA）CCHE1在乳腺癌组织中的表达及其临床意义。
方法收集本院2011年1月至2012年12月切除的乳腺癌组织115例和配对癌旁组织78例，采用实时荧光定量PCR（QPCR）检测CCHE1在上述组织中的表达情况，比较乳腺癌组织和癌旁组织中的CCHE1水平；分析CCHE1水平与乳腺癌临床病理参数（年龄、临床T分期、组织学分级、淋巴结转移、Nottingham预后指数、Ki67增殖指数、ER表达、PR表达及HER2扩增）和复发的关系；分析不同CCHE1水平的总生存期（OS）和无进展生存期（PFS），采用Cox比例风险模型进行多因素分析。
结果QPCR检测发现，115例乳腺癌组织中的CCHE1水平为7610±3210，高于癌旁组织的2142±1753（P＜005）。CCHE1水平与临床T分期、淋巴结转移、Nottingham预后指数、HER2扩增和肿瘤复发有关（P＜005），而与年龄、组织学分级、ER表达、PR表达和Ki67增殖指数无关（P＞005）。CCHE1低表达组的中位PFS和OS分别为560个月和630个月，均优于高表达组的370个月和420个月（P＜005）；CCHE1水平、临床T分期、淋巴结转移、Nottingham预后指数和HER2扩增是影响OS和PFS的独立预后因素（P＜005）。
结论CCHE1在乳腺癌组织中表达升高，该lncRNA可能在乳腺癌的发生、发展中有一定作用，可作为潜在地评估乳腺癌患者预后的分子标志物。

关键词: 乳腺癌, 长链非编码RNA, CCHE1, 预后

Abstract: ObjectiveTo investigate the expression of long noncoding RNA（lncRNA） CCHE1 （cervical carcinoma highexpressed 1） and its clinical significance in breast cancer tissues.
MethodsOne hundred and fifteen cases of breast cancer tissues and 78 cases of paired paracancerous tissues were collected in our hospital from January 2011 to December 2012. The expression of CCHE1 was detected in the above tissues by realtime fluorescence quantitative PCR （QPCR）. CCHE1 levels in breast cancer tissues and adjacent tissues were compared. The relationship between the CCHE1 level and the clinicopathological parameters of breast cancer （age， clinical T stage， histological grade， lymph node metastasis， Nottingham prognostic index， Ki67 proliferative index， ER expression， PR expression and HER2 amplification） and recurrence were analyzed. The overall survival （OS） and progressionfree survival （PFS） at different CCHE1 levels were analyzed， and the Cox proportional hazards model was used for multivariate analysis.
ResultsQPCR detection showed that the level of CCHE1 in 115 cases of breast cancer tissues was 7610±3210， higher than 2142±1753 in 78 cases of adjacent tissues （P＜005）. The level of CCHE1 was significantly related with clinical T staging， lymph node metastasis， Nottingham prognosis index， HER2 amplification and tumor recurrence （P＜005）， but not related to age， histological grade， ER expression， PR expression and Ki67 proliferative index （P＞005）. The median PFS and OS of the CCHE1 lowexpression group were 560 months and 630 months， better than 370 months and 420 months of the CCHE1 highexpression group （P＜005）. The CCHE1 level， clinical T stage， lymph node metastasis， Nottingham prognostic index and HER2 amplification were independent prognostic factors of OS and PFS （P＜005）.
ConclusionThe increased expression of CCHE1 in breast cancer tissues may play a role in the development of breast cancer， and this lncRNA can be a potential molecular marker for evaluating the prognosis of breast cancer.

Key words: Breast cancer, Long noncoding RNA, Cervical carcinoma highexpressed 1 （CCHE1）, Prognosis

熊晶, 王渝, 王君, 周晟. 乳腺癌组织中lncRNA CCHE1水平及其临床意义[J]. 临床肿瘤学杂志, 2018, 23(6): 519-523.

XIONG Jing, WANG Yu, WANG Jun, ZHOU Sheng. Expression of lncRNA CCHE1 and its clinical significance in breast cancer tissues[J]. Chinese Clinical Oncology, 2018, 23(6): 519-523.

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