Abstract: ObjectiveTo investigate the level of microRNA21（miR21） in glioma tissues and its relationship with the expressions of epidermal growth factor receptor（EGFR） and vascular endothelial growth factor（VEGF）. 
MethodsNinetytwo cases of glioma tissues（WHO Ⅲ and Ⅳ grade） surgically resected and confirmed by pathology in our hospital from January 2005 to June 2015 were collected. Another 50 cases of paracancerous tissues were taken as control. Realtime quantitative PCR（QPCR） was used to detect the level of miR21 in the above tissues. Immunohistochemistry was used to detect the expressions of EGFR and VEGF. The relationships between miR21 level and clinicopathological parameters（gender， age， nationality and pathological grade） as well as the expressions of EGFR and VEGF were analyzed in glioma tissues. Correlation between miR21 level and expressions of EGFR and VEGF was analyzed by Spearman rank correlation analysis. 
ResultsQPCR detection showed that the miR21 level in glioma tissues was 1307±769， higher than that in adjacent tissues， and the difference was statistically significant（P＜005）. The level of miR21 was not related to sex， age and nationality（P＞005）， only related to the pathological grade（P＜005）. The level of miR21 of grade Ⅳ was 16483±4980， higher than 3229±3165 of grade Ⅲ， and the difference was statistically significant（P＜005）. The positive expression rates of EGFR and VEGF were both 87％（80／92）. The miR21 level in EGFR positive ones was 13952±8062， higher than 3487±3309 of the negative ones（P＜005）； the miR21 level of the VEGF positive ones was 13874±8340， higher than 2090±1458 of the negative ones（P＜005）. Spearman rank correlation analysis showed that the miR21 level in glioma tissues was positively correlated with the expressions of EGFR and VEGF（r=0251， 0311， P＜005）. 
ConclusionThe level of miR21 in glioma tissues was elevated， which was related to the pathological classification and the expression of EGFR and VEGF. It may be involved in the development of the malignant tumor， and it has certain clinical significance for the diagnosis and treatment of glioma.

Key words: Glioma, MicroRNA21, Epidermal growth factor receptor, Vascular endothelial growth factor