Chinese Clinical Oncology

The effect of miR-203 on the migration and invasion of lung adenocarcinoma cells via targeting Bmi-1

XU Lei， JIANG Feng， YANG Xin， DA Liangshan， QIAN Yichun， WANG Jie， YIN Rong， XU Lin

Chinese Clinical Oncology. 2014, 19 (4): 289.

Abstract ( 960 )

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Objective To investigate the expression of miR203 in lung adenocarcinoma and analyze the relationship between miR-203 and migration and invasion of lung adenocarcinoma cells. The involved molecular mechanisms are also initially explored. Methods miR-203 was detected in lung tissues of 40 patients with lung adenocarcinoma by real time PCR. The expression of miR-203 was detected in lung adenocarcinoma cell lines H1650， A549， H1975， SPC-A-1 by real time PCR. The potential target gene of miR203 was predicted by online bioinformatic softwares. Pre-miR-203 mimics， Bmi-1 gene and Bmi-1 siRNA were transfected into H1975 cell line by lipofectamine 2000. The Bmi-1 protein level was analyzed by Western blotting. The predicted miR-203 binding site in Bmi-1 3’untranslated region（UTR） was validated by dualluciferase reporter gene assay. The migration ability of H1975 cells was determined by Transwell assay.
Results The relative expression of miR-203 in lung adenocarcinoma tissues was 0.065±0.013. The expression level of miR-203 in the lymph node metastasis group was lower than those in the non-metastasis group. The relative expression of miR-203 in H1650, A549，H1975 and SPC-A-1 cell lines were 0.280±0.102，0.308±0.168，0.167±0.073 and 0.287±0.096， respectively. Bmi-1 was a potential target gene of miR-203 predicted by miRanda and TargetScan. The Bmi-1 protein level was remarkably decreased in the pre-miR-203 mimics group. Dual-luciferase reporter gene assay validated the predicted miR-203 binding site of Bmi-1 3’UTR. Overexpression of miR-203 significantly inhibited the migration and invasion of H1975 cells， whereas the cell migration and invasion ability could be restored by overexpression of Bmi-1. Conclusion miR-203 can suppress the migration of lung adenocarcinoma cell line H1975 via downregulating Bmi-1 expression. miR-203 might be a potential tumor metastasis suppressor miRNA.

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Influence of FAM21 on migration and invasion properties of the choriocarcinoma cell line JEG-3

NI Xiaobei， LI Xin， LING Xiufeng， HUO Ran

Chinese Clinical Oncology. 2014, 19 (4): 294.

Abstract ( 1173 )

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Objective To explore the influence of FAM21 on migration and invasion properties of choriocarcinoma cell line JEG-3. Methods Two FAM21specific small interfering RNA（siRNA1 and siRNA2） and one scramble siRNA（control siRNA） were designed. According to the experimental design， the JEG-3 cells with stable transfection of siRNA1， siRNA2 and control siRNA were assigned into three groups： siRNA1 group， siRNA2 group and control siRNA group. The JEG-3 cells without transfection were selected as blank（blank group）. FAM21 mRNA levels of JEG-3 cells and human trophoblast HTR-8/Svneo cells were detected by normal PCR. The quantitative PCR and Western blotting were employed to measure the FAM21 mRNA level at 24h after transfection and protein level at 48h after transfection of each group， respectively. The migration and invasion properties of each group at 24h after transfection were detected by Transwell method. The quantitative PCR was employed to detect the mRNA levels of invasion-related genes（KISS-1， BRMS1 and INSL4） at 24h after transfection. Results There was a higher mRNA level of FAM21 in JEG-3 cells versus HTR-8／Svneo cells （P＜0.05）. The FAM21 mRNA level at 24h and FAM21 protein level at 48h after transfection of siRNA1 and siRNA2 groups were lower than those of control siRNA group and blank group with significant differences（P＜0.05）. There were lower migration and invasion properties in siRNA1 and siRNA2 groups versus control siRNA group and blank group at 24h after transfection（P＜0.05）. There were decreased mRNA levels of KISS-1 and BRMS1 and increased level of INSL4 in siRNA1 and siRNA2 groups versus control siRNA group and blank group at 24h after transfection（P＜0.05）. No significant difference was observed on the above indicators between control siRNA group and blank group（P＞0.05）.
ConclusionFAM21 protein can regulate the migration and invasion properties of the choriocarcinoma cell line JEG-3， which plays an important role in the pathogenesis of choriocarcinoma.

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Study on the efficacy with sodium cantharidinate against pancreatic cancer cells

SHOU Liumei，TAO Min

Chinese Clinical Oncology. 2014, 19 (4): 299.

Abstract ( 1084 )

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Objective To investigate the potential anti-proliferative effect of sodium cantharidinate on pancreatic cancer cells PANC-1，CFPAC-1 in vitro and explore its possible anti-cancer mechanism.
Methods Different concentration of sodium cantharidina（10，20，30，40，50μmol／L）were added to PANC-1 and CFPAC-1 cells for 24, 48h. The MTT assay was performed to reveal the inhibitory effect on cell proliferation. Clone formation ability was determined by flat plate clone formation assay. Cell cycle was tested by flow cytometry using PI staining.
Results Sodium cantharidinate with different concentration treatment inhibited the proliferation in a dose and time-dependent manner. Meanwhile， Sodium cantharidinate（10μmol／L） repressed pancreatic cancer cells clone formation and induced cell cycle arrest at G2/M phase. The percentage of G2/M cell cycle in PANC-1 cells increased from （24.75±1.08）% to （35.68±1.84）%, from （28.88±1.66）% to （36.34±1.25）% in CFPAC-1 cells.
Conclusion Sodium cantharidinate has obvious cytotoxic efficacy and inhibit the growth to human pancreatic cancer cel1s PANC-1 and CFPAC-1. The inhibition mechanism can be associated with arresting cell cycle at G2/M phase.

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Expression of IGF-IR and SUSD3 in breast cancer and their clinical significance

YU Zhenghong，WANG Xinxing， SHI Yaqin， GU Jun， JIANG Enze，YU Bo， ZHANG Xinhua， CHEN Longbang.

Chinese Clinical Oncology. 2014, 19 (4): 303.

Abstract ( 1128 )

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Objective To investigate the expression of insulin-like growth factorI receptor （IGF-IR） and sushi domaincontaining protein 3 （SUSD3） in breast cancer tissue， and analyze their relationship with clinical parameters and the correlation between the two proteins. Methods The expression of IGF-IR and SUSD3 in 100 cases of beast cancer tissues and the corresponding adjacent normal breast tissues after surgery was detected by immunohistochemical technique MaxVisionTM， and the relationship with clinical pathological features was further analyzed.
Results The positive rate of IGF-IR protein was 86.0％ in breast cancer tissues， higher than 3.0％ in adjacent normal breast tissues（P＜0.05）.The positive expression rate of SUSD3 protein was 780％ in breast cancer tissues， higher than 2.0％ in adjacent normal breast tissues（P＜0.05）. The expression of IGF-IR and SUSD3 was related to estrogen receptor and pathological types （P＜0.05）， but not with age， stage， the expression of HER-2 and Ki-67（P＞0. 05）. The expression of IGFIR and SUSD3 in breast cancer tissue was positively related （r=0.553， P＜0.01）.
Conclusion The expression of IGF-IR and SUSD3 may be correlated to the occurrence and development of breast cancer. The combined detection of IGF-IR， SUSD3 and ER may play an important role in judging prognosis and guiding adjuvant therapy after surgery of breast cancer.

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Expression of MIF， IL-17 and IL-10 in hepatocellular carcinoma and their clinical significance

ZHAO Xiaping， LI Xiaoyun， YU Xiaohui.

Chinese Clinical Oncology. 2014, 19 (4): 307.

Abstract ( 1099 )

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Objective To detect the expression of macrophage migration inhibitory factor（MIF）， interleukin（IL）-17 and IL-10 in hepatocellular carcinoma（HCC）， and discuss their clinical significance.
Methods Fifty-two cases of HCC tissues， the adjacent liver cirrhosis（LC） tissues of HCC and 20 cases normal liver samples in the department of hepatobiliary surgery, Lanzhou General Hospital, Lanzhou Command, from September 2010 to March 2013 were collected. Immunohistochemistry technique was used to detect the expression of MIF， IL-17 and IL-10 in tissues of three groups. The relationship between the expression and clinicopathological features of HCC， such as the tumor size， degrees of differentiation and other features were analyzed.
Results The positive rates of MIF， IL-17and IL-10 were 78.8％，76.9％，28.8％ in HCC tissues respectively；75.0％，73.0％，32.7％ in LC tissues；and 20.0％，25.0％，70.0％ in the normal liver tissues. Compared with the normal liver tissue， the MIF and IL-17 expression of HCC and LC tissues were higher， and IL-10 expression was lower， with statistical significances （P＜0.05）. The expression of MIF， IL-17 and IL-10 in HCC tissues was related to the tumor size， degrees of differentiation， not related to age， gender， tumor thrombus and ChildPugh stage. The different expression of MIF, IL-17, IL-10 in HCC tissues was related to one year survival rate of HCC patients.
Conclusion The expression of MIF， IL-17 and IL-10 are closely correlated with the occurence, development and prognosis of HCC.

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The correlation between single nucleotide polymorphism in XRCC1 and clinical response to platinum-based chemotherapy in ovarian cancer

CHENG Li， LI Lin， XING Hui.

Chinese Clinical Oncology. 2014, 19 (4): 312.

Abstract ( 1036 )

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Objective To investigate the relationship between XRCC1 Arg194Trp and Arg399Gln polymorphism genotype and chemosensitivity to platinum-based in ovarian cancer.
Methods Polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP） analysis was performed to detect 82 cases of XRCC1 Arg194Trp and Arg399Gln polymorphism genotype after platinumbased chemotherapy drugs up to six cycles of ovarian cancer. And the association between the two genes sites were analyzed.
Results XRCC1 Arg194Trp had three genotypes， namely Arg／Arg， Arg／Trp， Trp／Trp， and frequency distribution was 47.6％， 43.9％， 8.5％， respectively. XRCC1 Arg399Gln had three genotypes， namely Arg／Arg， Arg／Gln， Gln／Gln， and frequency distribution was 25.6％， 40.2％， 34.1％， respectively. There were statistically significant difference of different genotypes in FIGO stage and age group （P＜0.05）. The genotypes existed difference between the group of sensitive to chemotherapy and nonsensitive to chemotherapy （P＜0.05）. XRCC1 Arg194Trp Trp／Trp genotype and Arg399Gln Gln/Gln genotype were easier to resistance to platinum drug compared with the Arg／Arg genotype， the odds ratio could be increased by 13.50 （95％CI： 1.461-124.739） times and 7.65 （95％CI： 2.012-29.088） times, respectively. While carrying 194Arg／Trp and 399Gln／Gln patients have 84.62％ of non-sensitive to chemotherapy（OR=22.00，95％CI：2.534～190.998；P＜0.05）. Conclusion Polymorphism in the XRCC1 Arg194Trp and Arg399Gln may have significant impact on the response of ovarian cancer to platinum-based chemotherapy； and the two gene sites have combined effect.

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Clinical study on high-dose interferon alpha-2b adjuvant treatment in Chinese oral mucosal malignant melanoma

WANG Runxiang， WU Yunteng， LI Chaojun， LV Jiong， GUO Wei， REN Guoxin.

Chinese Clinical Oncology. 2014, 19 (4): 318.

Abstract ( 959 )

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Objective To investigate the therapeutic effects and main side effects of highdose interferon alpha-2b（IFN-α-2b） adjuvant treatment in Chinese oral mucosal melanoma. Methods A total of 117 patients with oral mucosal melanomas with stage ⅢⅣA after surgery from May 2004 to November 2012 were analyzed retrospectively. In the 117 cases of oral mucosal melanoma， 73 cases received the adjuvant therapy of high-dose IFN-α-2b， of whom 58 cases finished the treatment（treatment group） and 15 cases discontinued treatment. The rest 44 cases who didn't receive high-dose IFN-α-2b adjuvant treatment were selected as the control group. The overall survival（OS）， relapsefree survival（RFS） and side effects between the two groups were compared. Results The median OS of 117cases was 40 months（95％CI：33-62months）. For the patients with stage Ⅲ， the differences of median OS（69 months vs.65 months） and RFS（50 months vs.34 months） were not significant between the two groups. While those of cases with stage ⅣA had significant differences， the median OS was 37 months vs. 20 months（P=0028）， and the RFS was 33 months vs. 10 months（P=0.004）. Furthermore， the benefit of treatment was greater for those of cervical lymph nodes involved in the patients with stage ⅣA. The median OS was 40 months vs. 20 months, the median RFS was 33 months vs. 10 months. Adverse reactions occurred in most patients treated with highdose IFN-α-2b. The common hematology toxicity was bone marrow suppression， including low counts of white blood cells and platelets. The other sideeffects included druginduced influenzalike syndrome， gastrointestinal responses and liver function damage. Toxicity of grade 1-2 was predominant， only 7 cases had grade 3-4 toxicity. After given symptomatic treatment， all of them could be relieved. No treatment-related death happened. Conclusion High-dose IFN-α-2b adjuvant treatment may improve overall survival time as well as relapse-free survival time in patients with advanced oral mucosal melanoma（stage ⅣA），and the side effects are tolerable.

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Prognostic analysis of 132 cases with tongue squamous cell carcinoma in Xinjiang

WANG Boqing， LIU Pan， ZHAO Huarong， ZHANG Songan， Huerxidan Niyazi， BAO Yongxing.

Chinese Clinical Oncology. 2014, 19 (4): 323.

Abstract ( 868 )

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Objective To explore the prognostic factors for 132 cases with tongue squamous cell carcinoma（TSCC）in Xinjiang.
Methods One hundred and thirtytwo patients with pathologically confirmed TSCC admitted by the first affiliated hospital of Xinjiang medical university from Jan 2003 to Dec 2011 were enrolled. There were 108 cases of surgery（94 with routine cervical lymph node dissection）and 24 cases without surgery. According to the treatment protocols， 29 cases were treated with surgery alone， 10 cases with radiotherapy alone and 93 cases with comprehensive treatment. The patients were followed up and overall survival was estimated using the Kaplan-Meier method. The 5-year survival rates of different clinicopathological parameters（gender， ethnicity， age， degree of differentiation， T stage， N stage， TNM stage， invading the tongue root， invading the midline， invading the mouth floor and local recurrence）and treatment protocols（primary lesion resection， radiotherapy， chemotherapy， cervical lymph node dissection and surgical situation） were investigated. The Cox regression was employed to analyze the independent prognostic factors. Results Of the 132 patients， 9 were lost during the followup periods ranging from 5 to 120 months with 61 alive and 62 dead（58 of cancerrelated diseases）. The median overall survival time was 800 months and 5year survival rate was 598％. The 5year survival rates varied among different degree of differentiation， T stage， N stage， TNM stage， invading the midline， invading the mouth floor and local recurrence or primary lesion resection， radiotherapy， cervical lymph node dissection and surgical situation. Degree of differentiation， lesion resection， local recurrence， TNM stage and radiotherapy were independent factors affecting the prognosis.
Conclusion Curative resection of the primary lesion， rational cervical lymph node dissection and prevention of local recurrence may improve the prognosis of patients with tongue squamous cell carcinoma. Surgery alone can provide better prognosis for stage Ⅰ-Ⅱ， and comprehensive therapy including surgery can give better prognosis for stage Ⅲ-Ⅳ.

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A meta-analysis of association between EPHX1 A415G polymorphism and digestive tract cancer susceptibility

WANG Dongfeng，LIU Wei，ZHANG Dong，XU Fapei，ZHONG Jian，YAO Chengyun，SUN Jingfeng.

Chinese Clinical Oncology. 2014, 19 (4): 329.

Abstract ( 944 )

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Objective To explore the correlation between polymorphism of microsomal epoxide hydrolase 1（EPHX1） A415G and susceptibility to gastrointestinal tumors.
Methods All eligible casecontrol studies published up to May 2013 were searched out from PubMed， EMABSE， CBM， VIP, WanFang and CNKI databases. Two reviewers independently identified the literature, extracted data and assessed the quality of included studies according to inclusion and exclusion criteria. Metaanalysis was performed using STATA 11.0 software to analyze. Results A total of 18 studies comprising 5852 patients and 8710 controls were finally included. The included studies showed good homogeneity in the four genetic models of GG vs. AA， GA vs. AA，GG/GA vs. AA and GG vs. GA/AA. Overall， there was no significant association between polymorphism of EPHX1 A415G and susceptibility to gastrointestinal tumors［GG vs. AA： OR=1.063， 95％CI： 0.888-1.273； GA vs. AA： OR=0.935， 95％CI： 0.867-1.009；GG/GA vs. AA： OR=0.948， 95％ CI： 0.882-1.020； GG vs. GA/AA： OR=1.091， 95％ CI： 0.913-1.304］. Conclusion This metaanalysis suggest that there is no correlation between polymorphism of EPHX1 A415G and susceptibility to gastrointestinal tumors.

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奥氮平预防化疗所致爆发性呕吐的临床观察

PANG Hongxia， ZHENG Weisheng， KE Qiuqing， HONG Yanni， HONG Yaping

Chinese Clinical Oncology. 2014, 19 (4): 334.

Abstract ( 1100 )

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Objective To evaluate the efficacy and safety of olanzapine in prevention of breakthrough chemotherapyinduced vomiting in cancer patients receiving highly emetogenic chemotherapy.
Methods Thirty-six cancer patients with chemotherapy related breakthrough vomiting in the first cycle were enrolled in this study. These patients were given olanzapine（10mg once daily, d1-d3）orally before the second cycle of chemotherapy to prevent explosive vomiting. The nausea and vomiting of patients were scored according to the national cancer institute common terminology criteria for adverse events. The test was used to compare the differences of vomiting scores before and after receiving olanzapine. Results The vomiting scores of the 36 cases were significantly decreased （10.1±0.9 vs. 18.2±1.2， P=0.032） after combined with olanzapine in prevention. 47.2％（17/36） of the patients no longer had explosive vomiting after receiving olanzapine and 75.0％（9／12） of women patients no longer had vomiting after receiving olanzapine. The effective rate of olanzapine in prevention of vomitting was 80.0%. The main related side effects were dizziness and fatigue， and the incidences were 36.1％ and 30.6％， respectively. Conclusion Olanzapine can effectively reduce the incidence of chemotherapy related breakthrough vomiting， and release the symptom of nausea and vomiting in cancer patients.

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Comparison of different surgical treatment of retroperitoneal laparoscopic partial nephrectomy for patients with localized renal cell carcinoma

GONG Jun， XU Chuanliang， REN Shancheng， GE Jingping， WEI Wu， YANG Bin， SUN Yinghao.

Chinese Clinical Oncology. 2014, 19 (4): 338.

Abstract ( 1068 )

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Objective To investigate the efficacy and safety of different surgical treatment of retroperitoneal laparoscopic partial nephrectomy for patients with localized renal cell carcinoma.
Methods 127 patients with tumor size ＜4cm underwent RLPN from February 2006 to December 2012 in our hospital. According to the course of the new surgical techniques adopted， they were divided into 4 groups：conventional group（n=44）， simple enucleation group（n=35）， knotfree suture group（n=39）and selective artery clamping group（n=9）. The data of operative time， renal artery clamping time， blood loss during operation， postoperative hospital stay， intraoperative transfusion rate and the incidence of urinary leakage postoperation were collected， and those data of the former three groups were statistical analyzed.
Results The mean time of renal artery clamping in the former three groups was（32.07±5.59）min，（30.20±5.84）min，（27.31±6.17）min，respectively. The mean operative time were（109.68±20.07）min，（106.20±16.32）min，（97.00±17.65）min，respectively. The mean blood loss during operation was（106.93±72.26）ml，（80.26±49.57）ml，（54.23±36.32）ml，respectively. The mean time of postoperative hospital stay was（7.82±1.42）d，（6.31±1.69）d，（5.97±1.51）d， respectively. There were statistically differences between the three groups on the time of renal artery clamping， the operative time， the blood loss and the time of postoperative hospital stay（P＜005）. The rate of transfusion during operation was 2.3％（1／44）， 0（0／35）， 0（0／39）， respectively. The incidence of urinary leakage after operation was 0（0／44）， 2.9％（1／35）， 2.6％（1／39）. The above clinical parameters had no significant differences among the three groups（P＞0.05）. All the patients were followed up for 1460 months, and no recurrence and metastasis was found.
ConclusionFor small renal cell cancer, the techniques of simple enucleation and knot free suture can significantly reduce renal artery clamping time and blood loss during operation. The techniques of selective artery clamping will prolong the operation time and almost have no warm ischemia time and it can deserve further research.

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The correlation between the contrastenhanced ultrasound and pathological differentiation in small hepatocellular carcinoma

CHEN Qiong， SI Qin， QIAN Xiaoli， HUANG Shengxi， YANG Lu， LIU Yuanyuan.

Chinese Clinical Oncology. 2014, 19 (4): 342.

Abstract ( 1078 )

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Objective To investigate the relationship between the contrastenhanced ultrasound perfusion characteristics of small hepatocellular carcinoma（SHCC） and pathology and its clinical value.
Methods Three hundred and sixtynine cases liver focus lesions detected by conventional ultrasound were examined by contrastenhanced ultrasound（CEUS）. The CEUS imaging characteristics and pathological results of SHCC were analyzed and compared. Results（1） Compared with pathological results of 254 malignant lesions and 124 benign lesions，the sensitivity of CEUS in diagnosis of SHCC was 98.8％（242／245）， the specificity was 92.7％（115／124）， the accuracy was 96.7％（357／369）， Jorden index was 0.92， the positive likelihood ratio was 13.7， negative likelihood ratio was 0.013.（2） The CEUS characteristics of SHCC were rapid enhancement in early artery period， rapid clearance in late artery period or early portal period， that was characterized by fast enhancement and clearance. The enhancement pattern of fast in and out was accounted for 87.2％（219／251） in malignant tumor lesions compared with 1.7％（2／118） in benign lesions. The difference has statistical significance（P＜0.05）.（3） The differences of CEUS enhancement time and peak time in different pathological differentiation of SHCC were no statistically significant（P＞0.05）， but the differences of time to clarify were statistically significant（P＜0.05）. Clearance time of the high differentiation，moderate differentiation and low differentiation of SHCC was（102.4±2.36）s，（76.02±3.88）s，（49.40±4.95）s， respectively. The clearance time in high differentiation of SHCC was significantly longer than that of the low differentiation degree of SHCC（P＜0.05）.
ConclusionCEUS has important clinical value in the diagnosis of SHCC and the evaluation of pathological differentiation degree.

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The clinical value of autofluorescence bronchoscopy for the diagnosis of central type early lung cancer

ZHENG Xiaoxuan， SUN Jiayuan， HAN Baohui.

Chinese Clinical Oncology. 2014, 19 (4): 346.

Abstract ( 1062 )

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Objective To investigate the value of using autofluorescence bronchoscopy（AFB） for the detection and localization of central type early lung cancer. Methods 93 patients，suspected of lung cancer were examinated by AFB and white light bronchoscopy（WLB）. All of the suspicious abnormal sites were recorded and biopsied for pathological examination. Two methods were compared by sensitivity and specificity. Results Included 93 biopsy specimens，and 74 were classified as positive by pathology，including 70 invasive cancer，1 carcinoma in situ and 3 severe dysplasia. AFB combined with WLB found positive resuts comfirmed by pathology in 67 cases, and WLB alone found positive in 50 cases. The relative sensitivity to detect central type early lung cancer of WLB combined with AFB versus WLB alone was 90.5％ and 67.6％, respectively，with statistical difference significantly（P＜0.05）. Conclusion The sensitivity of AFB is better than WLB in detection and localization of central type early lung cancer.

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Clinical observation of intrapleural or intraperitoneal hyperthermic perfusion plus bevacizumab on malignant pleural or peritoneal effusion

KANG Huanrong， JIANG Jian， DU Nan.

Chinese Clinical Oncology. 2014, 19 (4): 350.

Abstract ( 1177 )

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Dose escalation trial of lobaplatin combined with adriamycin and ifosfamide for neoadjuvant chemotherapy in osteosarcoma

HEN Guojing， WANG Zhen，YE Xinhong， WU Zhigang， ZHU Haodong， SHI Lei， FU Jun.

Chinese Clinical Oncology. 2014, 19 (4): 354.

Abstract ( 1146 )

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Objective To investigate the maximum-tolerated dose（MTD） and observe the side effects of lobaplatin（LBP） combined with adriamycin（ADM） and ifosfamide （IFO） for neoadjuvant chemotherapy in osteosarcoma.
MethodsThe doses of ADM and IFO were fixed（ADM 60mg／m²，IFO 12g／m²），and three dose levels of LBP were setted， namely 45mg／m²， 50mg／m²， 55mg／m². The dose of LBP would increase gradually until doselimiting toxicity（DLT） or 55mg／m². Results Six cases were enrolled， and 2 DLT cases were observed when the test to the first dose group. So the MTD was LBP 45mg／m2， ADM 60mg／m²， IFO 12g／m². The main toxicities were reversible bone marrow suppression. The incidence rate of thrombocytopenia was 66.6％（4／6） and grade 3 was 33.3％（2／6）. The incidence rate of leukocytopenia was 83.3％（5／6） and no grade 3-4 toxicity was observed 0 （0／6）. The incidence of neutropenia was 83.3％（5／6） and grade 4 was 33.3％（2／6）. All patients were recovered by symptomatic treatment. Conclusion The MTD of LBP combined with ADM and IFO as neoadjuvant chemotherapy in treating osteosarcoma is LBP 45mg／m2， ADM 60mg／m²， IFO 12g／m². The therapy is well tolerated and recommended as a basis for further clinical studies.

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Expert consensus of comprehensive diagnosis and treatment of pancreatic cancer in China(version 2014)

Chinese Clinical Oncology. 2014, 19 (4): 358.

Abstract ( 644 )

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Progress on correlation between Mfn2 and RasMAPK signal pathway in breast cancer

SHAN Xijin， ZHANG Jinghua， LI Yufeng， LIU Yan.

Chinese Clinical Oncology. 2014, 19 (4): 371.

Abstract ( 1075 )

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Mitochondrial fusion protein 2（Mfn2）is a highly conserved transmembrane GTP enzyme， which is encoded by a newly identified tumor suppressor gene Mfn2. The antitumor effect of Mfn2 is associated with the mitogenactivated protein kinase（MAPK）signaling pathway. MAPK is a major downstream tributary of Ras signaling pathway， which plays an important role in the malignant cells， leading to the tumor invasion and metastasis. In this review， we focus on the recent discoveries on the mechanisms involved in the regulation of Mfn2 and the relationship between Mfn2 and RasMAPK signaling pathway in breast cancer.

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Research advance in gamma delta T cells and its antitumor activity

SU Dan，WANG Dongliang，ZHANG Yanqiao

Chinese Clinical Oncology. 2014, 19 (4): 375.

Abstract ( 1615 )

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Gamma delta（γδ） T cells are minor subset of T lymphocytes（2％-5％ of peripheral blood T lymphocytes） that express Vγ9Vδ2 T cell receptors， mainly distributed in the mucosaassociated lymphoid tissue, are nonrestricted cells of major histocompatibility complex（MHC）， can kill various tumor cells. Many data demonstrate that γδ T cells are involved in the first line of immune defense system. In the future， adoptive γδ T cellsbased cell immunotherapy will become a new strategy for cancer immunotherapy. In this review， we provide an overview of its antitumor mechanism and clinical application.

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Progress of drugs in nonsmall cell lung cancer with brain metastasis

CAI Zhongfu，OUYANG Xuenong，YU Zongyang.

Chinese Clinical Oncology. 2014, 19 (4): 379.

Abstract ( 1096 )

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Non-small cell lung cancer（NSCLC）is one of the most common malignant tumor and a major cause of death from cancer. NSCLC patients often poorly responds to chemotherapy and has a relatively poor prognosis，especially in those patients with brain metastases. NSCLC patients with brain metastases have poor prognosis and worse quality of life； the natural median survival time is only 1-2 months. To some extent，whole brain radiation therapy improves quality of life and prolongs the survival time in NSCLC patients with brain metastases； but the curative effects are still unsatisfactory.In recent years，some research around pemetrexed，temozolomide and epidermal growth factor receptortyrosine kinase inhibitors（EGFR-TKIs）are conducted and achieves certain results. In this paper，we review the progress of drugs in NSCLC with brain metastasis.

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标准刊号：	ISSN 1009-0460
	CN 32-1577/R