Objective To observe the effect of NRP-1b1／b2 IgG monoclonal antibody （NRP-1mAb） on the growth of gastric cancer cell BGC-823， and to explore the possible mechanism of the antibody. Methods NRP-1mAb was prepared in laboratory， and the purity of antibody was detected by SDS-PAGE. Gastric cancer BGC-823 cells were cultured by 0， 25， 100， 200， 400 μg／ml NRP-1 mAb. The morphological changes of BGC-823 cells were observed by microscope. The proliferation， colony formation and apoptosis of gastric cancer BGC-823 cells were observed by MTT assay， colony forming assay and flow cytometry. The phosphorylation of related signal proteins was detected by Western blotting analysis. Results SDS-PAGE test showed that the purity of NRP-1mAb was above 95％. Microscopy showed apoptotic changes of BGC-823 cells treated by NRP-1mAb. MTT assay showed that NRP-1mAb could inhibit the proliferation of BGC-823 cells in a time and dose dependent manner（P＜0.01）. Colony forming assay showed that different doses of NRP1mAb could inhibit the colony formation of BGC-823 cells in a dose dependent manner（P＜0.01）. Flow cytometry showed that different doses of NRP-1mAb could promote the apoptosis of BGC-823 cells mainly at early apoptosis stage. It was found that the level of Akt phosphorylation was decreased after treated by NRP-1mAb， and there was no significant phosphorylation of ERK， p38 and JNK protein. Conclusion NRP-1mAb can inhibit the growth of gastric cancer cell BGC-823 and promote apoptosis， which may be related to the inhibition of Akt phosphorylation.

Objective To investigate the expression of acid-sensing ion channel 3（ASIC-3） and pain behavior in mice skin cancer pain after intrathecal amiloride.
MethodsBalb／c mice were randomly divided into 4 groups： Normal group （group N）， Cancer pain group （group C）， Saline group （group S） and Amiloride group （group A）. Each group was 10 mice. Except group N， the skin cancer pain model was set up through inoculating subcutaneously tumor cells into paw of mice. Group N and group C were not intervented by drugs. By intrathecal injection， the mice of group S were given 10 μl saline and mice of group A were given 15 μg/μl amiloride solution. Thermal withdrawal latency （TWL） of mice in each group on 0，30，60，120，180 minutes after temperature stimulation were detected respectively. At last， expression of ASIC-3 of spinal dorsal horn in mice was detected by Western blotting method. Results The TWL of each mice has no difference before inoculating（P＞0.05）. The TWL of group C，group S and group A was decreased from（9.66±0.54）s，（9.96±0.39）s，（10.15±0.40）s to （4.32±0.25）s，（4.16±0.33）s，（4.26±0.24）s after 2 weeks inoculation（P＜0.05）. On 60，120，180 minutes after injection， the TWL in group A was（6.03±0.23）s，（7.09±0.24）s，（6.49±0.19）s，which was higher than those of group C and group S（P＜0.05）. The relative expression of ASIC-3 in spinal cord of group A was 1.88±0.11， which was lower than that of group C（2.23±0.32） and group S（2.43±0.24）， and the difference was statistically significant （P＜0.05）. Conclusion Amiloride has the effect of inhibiting the ASIC-3 expression of the spinal dorsal horn in skin cancer pain mice， and taking part in the feeling of pain.

Objective To investigate the expression and clinical significance of long noncoding RNA（LncRNA） RAB11B-AS1 in gastric cancer. Methods The expression of LncRNA RAB11B-AS1 was detected by realtime quantitative PCR（QRT-PCR） in 83 cases of gastric cancer tissues and normal gastric tissues. The relationship of LncRNA RAB11B-AS1 expression with the clinicopathological features and prognosis of the patients was analyzed.
ResultsThe expression of LncRNA RAB11B-AS1 in gastric carcinoma tissue was 6.954±1.080， higher than 1.061±0.090 in normal gastric tissue， and the difference was statistically significant （P＜0.05）. The expression of LncRNARAB11B-AS1 was not related to sex， age and tumor location（P＞0.05）， but related to clinical stage， lymph node metastasis， distant metastasis， tumor differentiation and survival status （P＜0.05）. The median progression free survival （PFS） and overall survival （OS） of patients with high expression of LncRNA RAB11B-AS1 was 19.0 months and 29.0 months respectively， which was lower than 32.0 month and 430 months of patients with low expression of LncRNA RAB11B-AS1 （P＜0.05）. Cox multivariate regression analysis suggested that LncRNA RAB11B-AS1 expression， distant metastasis and clinical stage were independent prognostic factors of gastric cancer （P＜0.05）. Conclusion LncRNA RAB11BAS1 is involved in the regulation of gastric cancer， and can be used as a potential molecular marker for the diagnosis and prognosis of gastric cancer.

Objective To explore the expression of pololike kinase 1 （PLK1） and cell-division cycle protein 20 （CDC20） in breast cancer as well as the relationship of their expression with clinicopathological features and prognosis. Methods The expression datasets of PLK1 and CDC20 from cBioPortal database were collected to analyze the co-expressions of these two genes. The clinical data and mRNA microarray data of 1092 patients with breast cancer obtained from the Cancer Genome Atlas（TCGA） database were collected and analyzed for expression of PLK1 and CDC20. The KaplanMeier method and Cox proportional-hazards regression model was used to analyze the relationship between the expressions of PLK1 or CDC20 and the survival of patients with breast cancer. The relationships between PLK1 or CDC20 and age， gender and ER， PR or HER-2 genes expression levels were further analyzed by χ2 test. Results The expression levels of PLK1 and CDC20 in breast cancer were significantly correlated （r=0.88，P＜0.001）. Also， the expressions of PLK1 and CDC20 significantly was related to the disease free survival （DFS） of patients with breast cancer（P＜0.05）. Patients with high expression of PLK1 and CDC20 had short DFS compared with patients with low expression of both （P=0.007）. At the same time， the expression levels of PLK1 and CDC20 in ER negative and PR negative patients were significantly higher than those in ER positive and PR positive patients （P＜0.001）. Conclusion PLK1 and CDC20 may play a role in promoting tumorigenesis in breast cancer， and may serve as potential tumor diagnostic markers and individual therapeutic targets.

ObjectiveTo observe the efficacy and side effects of apatinib on advanced colorectal cancer patients， who failed in secondline and above chemotherapy. Methods Fourteen Ⅳ stage colorectal cancer patients who failed in secondline and above chemotherapy from January 2016 to November 2016 were enrolled in this study. Patients received the starting dose of 425 mg of apatinib per day. The efficacy and side effects were observed. Results Among the 14 advanced colorectal cancer patients， there were 2 cases of PR， 6 cases of SD and 6 cases of PD. The response rate was 14.3％，and the disease control rate was 57.1％. The median progressionfree survival was 78 days. The main side effects were hypertension， handfoot syndrome， mucositis， anorexia， leukopenia， thrombopenia and proteinuria， mainly in grade 1-3. Conclusion For the Ⅳ stage colorectal cancer patients who failed in secondline and above chemotherapy， apatinib 425 mg per day has good effect， and side effects can be tolerated.

Objective To explore the application of methylene blue and carbon nanoparticles suspension injection as lymphatic tracers in laparoscopic radical resection of cervical cancer. Methods Fifty patients diagnosed as cervical cancer from 2015 to 2016 were enrolled in this study， and were randomly divided into methylene blue group and carbon nanoparticles suspension injection group with 25 patients in each group. The detection rate of sentinel lymph nodes （SLN） of the two tracers was compared. Results The detection rate of SLN of methylene blue group and carbon nanoparticles suspension injection group was both 100.0％. In methylene blue group， 22 cases were detected with SLN. There were 48 SLNs in total with an average of 2.2， and the recognition rate was 88.0％. Five patients were found lymph node metastasis. In carbon nanoparticles suspension injection group， 23 cases were detected with SLN. There were 50 SLNs in total with an average of 2.3， and the recognition rate was 92.0％. Four patients were found lymph node metastasis. No obvious side effects were occurred. Conclusion Methylene blue and carbon nanoparticles suspension injection have fairly high recognition rate and detection rate for SLN in laparoscopic radical resection of cervival cancer, and they are safe and worthy of clinical application.

In recent years， the incidence of idiopathic pulmonary fibrosis in patients with lung cancer increased year by year. However， the diagnosis and treatment of idiopathic pulmonary fibrosis with lung cancer （IPF-LC） is difficult because of the lack of specific clinical manifestations， hematological and imaging features. IPF-LC patients often can not tolerate chemotherapy due to poor pulmonary function and other reasons， so the treatment options are limited. On the other hand，radiotherapy, chemotherapy and targeted treatment will increase the process of pulmonary fibrosis， leading to the treatment difficulties and poor prognosis. This study aims to summarize the epidemiology， pathogenesis， clinical features and treatment of IPF-LC.

Cancer， a group of various diseases involving unregulated cell growth， it is a lifethreatening disease with an alarmingly increased annual mortality rate globally. Although various therapies are used for cancer treatment, the effect is not satisfactory. Chemotherapy is one of the most commonly used mothods of treatment. However, severe adverse reactions and drug resistance always influence the chemotherapy effect. Ginseng is traditional Chinese medicine， ginsenoside Rg3 is the main pharmacologically active component in ginseng and has been reported to have an antitumor effect， which was originally used in ancient times for the treatment of diseases. This review sheds light on the molecular mechanisms underlying the cancer chemotherapeutic activity of ginsenoside Rg3 with particular focus on the mechanism associated with cell proliferation， apoptosis， angiogenesis， inflammation and the metastasis of cancer cells. The review can enhance the understanding of the anticancer mechanisms of ginsenoside Rg3， provide directions for clinical practice and give more information for developing novel anticancer agents with high efficiency， low toxicity and weak resistance.