Chinese Clinical Oncology

Inhibition effect of miR-7 on proliferation and metastasis of Hep-2 laryngeal carcinoma cells

XU Yao， OUYANG Xuenong， CHEN Xi， ZHAO Zhongquan， QI Xiaoyan.

Chinese Clinical Oncology. 2015, 20 (3): 193.

Abstract ( 953 )

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Objective To explore the effect of up-regulation of microRNA-7（miR-7） on proliferation and metastasis of Hep-2 laryngeal carcinoma cells and its possible mechanism. Methods The Hep-2 laryngeal carcinoma cells were divided into miR-7 group（Hep-2 cells transfected with miR-7 mimics）， Scramble group（Hep-2 cells transfected with miR-Scramble） and Mock group（Hep-2 cells without transfection）. The quantitive real-time PCR（qPCR） was used to test the expression of miR-7 at 48 h after transfection in 3 groups. The CCK-8 assay was used to test the proliferation of Hep-2 cells in each group at 48 h after transfection. Cell metastasis was determined by transwell test at 48 h after transfection. The mRNA and protein levels of PI3K（p110）， AKT and pAKT were examined by qPCR and Western blotting. Results The expression of miR-7 was significantly up-regulated after transfection with miR-7 mimics. The proliferation and metastasis of Hep-2 laryngeal carcinoma cells was extremely decreased after transfection with miR-7. The results from qPCR and Western blotting showed that the mRNA and protein levels of PI3K（p110）， AKT and pAKT were decreased in miR-7 group compared with other two groups（P＜0.05）. Conclusion Up-regulation of miR-7 can inhibit the proliferation and metastasis ability of Hep-2 laryngeal carcinoma cells with possible mechanism of inhibiting PI3K／AKT signal pathway.

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Expression of associated proteins in activited fibroblast in transplanted tumor in rat model

GUO Xiaodong， GUO Ruobing， CHEN Jie， WANG Jingxia， XIE Guoqun.

Chinese Clinical Oncology. 2015, 20 (3): 198.

Abstract ( 840 )

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Objective To observe expression difference of associated protein such as CD34, FAPα and α-SMA in activated fibroblasts among tissues of transplantation tumor in rat model， tissues adjacent to tumor and normal gastric tissues and to evaluate the role of fibroblast activated state in tumor growth. Methods Sixteen rats were randomly divided into model group（n=12） and normal control group（n=4）. Walker-256 cell lines were taken as the origin of rats model. Rat model was established by OB glue adhesive method. All rats raised at the same conditions. After 16 days， the tumor tissue， tissue adjacent to tumor and normal gastric tissue were removed. Expression of CD34， FAPα and α-SMA in these tissues was observed by immunohistochemical technique， Western blotting and RT-PCR. Results Compared with normal control group，expression of CD34 in tumor group and tissue adjacent to tumor group was significant lower（P＜0.05）. But expression of FAPα and α-SMA in these groups was higher than control group （P＜0.05）. There were different expression between tumor tissue and tissue adjacent to tumor in FAPα and α-SMA（P＜0.05）. Conclusion The different expression of CD34， FAPα and α-SMA in tumor tissue， tissue adjacent to tumor and normal gastric tissue indicated that there is correlation between fibroblasts activation and tumor development.

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Inhibition of metformin on acetyl coa carboxylase in human hepatocellular carcinoma cell line HepG2

PENG Xiaoren，LIU Yan，ZOU Dajin.

Chinese Clinical Oncology. 2015, 20 (3): 203.

Abstract ( 916 )

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Objective To determine the relationship between the antineopastic activity and the regulation on acetyl coa carboxylase （ACC） of metformin in human hepatocellular carcinoma cell（HCC） line HepG2. Methods HepG2 cells were treated with various concentrations of metformin（0，1，5，10，15 mmol／L） for 24 h， 48 h and 72 h respectively and cell growth was assessed by CCK8 assay. After treated with different doses of metformin（0，5，10，15 mmol／L） for 72 h， protein expression levels of AMPKα，P-AMPKα，ACC1，P-ACC were measured by Western blotting method and ACC mRNA expression levels were measured by Real-time PCR. Results The growth of HepG2 cells were inhibited by metformin in dosedependent and timedependent manner. After treated with metformin for 72 h， metformin increased AMPK activation and decreased ACC activation respectively as metformin dose levels increased. Compared with control group， the protein expression levels of P-AMPKα and P-ACC were both significantly changed in 10mmol／L group and 15 mmol／L group（P＜0.01）. ACC mRNA expression levels were decreased significantly in all metformin-treated groups（P＜0.01）. Conclusion Metformin inhibits cellular proliferation of HepG2 cell line and suppresses ACC activation in both aspects of protein phosphorylation and gene expression. Metformin actitiviates AMPK and inhibits ACC， which may implicate with its antineopastic activity on HCC.

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Effect and mechanism of scutellarein in suppressing the migration and proliferation of colorectal cancer HT-29 cells

ZHAO Xiaoyue， WANG Jiejun， YU Guanzhen， WANG Zhan， JIAO Xiaodong， LIU Ke， JIANG Longwei.

Chinese Clinical Oncology. 2015, 20 (3): 208.

Abstract ( 904 )

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Objective To explore the effect of scutellarein on the migration and proliferation of colorectal cancer HT-29 cells as well as the underlying mechanism. Methods The MTT assay was used to investigate the proliferation of HT-29 cells after treatment with different doses of scutellarein（5， 10， 20， 40， 80 μg／ml）. The effect of 80 μg／ml scutellarein on migration of HT29 cells was measured by Transwell assay. Protein expression of signal transducer and activator of transcription（STAT3）， nuclear factor（NF）-κB and p53 in HT-29 cells was assayed by Western blotting after treatment of 20， 40 μg／ml scutellarein. Cells without treatment were assigned as control group（0 μg/ml scutellarein）. Results Compared with the control group， scutellarein presented inhibitory effect on the proliferation ability of T29 cells in a dose-dependent manner with significant difference（P＜0.05）. There were decreased cell migration ability and protein levels of STAT3 and NF-κB but increased level of p53 in scutellarein group versus control group（P＜0.05）. Conclusion Scutellarein significantly inhibited the migration and proliferation of HT-29 cells with the possible mechanism of increasing expression of p53 and decreasing levels of STAT3 and NF-κB in HT-29 cells.

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Serum NTx in detecting bone metastasis and evaluating prognosis for lung cancer and breast cancer—a meta-analysis

QIAO Dan， WANG Zhiyu， WEN Xiaoting， LI Hongtao， ZHAO Hui.

Chinese Clinical Oncology. 2015, 20 (3): 212.

Abstract ( 896 )

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Objective To assess the value of serum crosslinked N-telopeptide of type I collagen （NTx） in the diagnosis of bone metastasis and prognosis of lung cancer and breast cancer. Methods PubMed， Cochrane Library， EMBase， CNKI， Wanfang database and VIP database were searched for collecting retrospective studies on serum NTx for diagnosis and prognosis of lung cancer and breast cancer. Studies were screened by the inclusion and exclusion criteria. QUADAS was used to evaluate the quality of the diagnostic studies. Stata 12.0 software was used to conduct the metaanalysis. Results Eleven literatures were selected， including 6 literatures in English and the others in Chinese， 8 studies about diagnosis， 2 studies about prognosis， and 1 study including diagnosis and prognosis were selected. A total of 1203 cases were identified， including 726 cases of lung cancer and 372 cases of breast cancer. The sensitivity and specificity of serum NTx in the diagnosis of bone metastasis from lung cancer and breast cancer were 76.5 ％（95％CI： 61.5％-86.9％） and 83.3％（95％CI： 77.1％-88.1％）， respectively. Merger HR estimate of overall survival for lung cancer and breast cancer patients with high serum NTx was 1.208 （95％CI： 1.128-1.293， P＜0.05）. Conclusion Despite the limitations of this study， serum NTx is a potential biomarker to detect bone metastasis and predict outcome in lung cancer and breast cancer. More prospective studies are necessary to analyze the clinical significance of serum NTx further.

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Expression and clinical significance of Cyclin A， PTEN and p27kip1 in esophageal squamous cell carcinoma

ZHAN Shudong， HUANG Junxing，YU Hong， WU Jing， ZHAO Guojun，YAO Juan， LU Ting， ZHOU Tongmin.

Chinese Clinical Oncology. 2015, 20 (3): 217.

Abstract ( 842 )

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Objective To investigate the expression and clinical significance of Cyclin A， PTEN and p27kip1 in esophageal squamous cell carcinoma （ESCC）.
MethodsThe expression of Cyclin A， PTEN and p27kip1 proteins were detected by the immunohistochemical En Vision method in 68 ESCC specimens and 25 tumoradjacent normal tissues. Results The positive expression rates of Cyclin A， PTEN and p27kip1 in esophageal squamous cell carcinoma were 60.3％， 35.3％， 41.2％，respectively，which had statistically significant difference with those of normal tissues （P＜0.05）. The expression of Cyclin A， PTEN and p27kip1 proteins were correlated with histological grade， lymph node metastasis and tumor size（P＜0.05）.Cyclin A expression was associated with age（P＜0.05）.The results of Kaplan-Meier survival analysis indicated that the positive expression of Cyclin A and the negative expression of PTEN and p27kip1were associated with a considerably worse survival. Conclusion Cyclin A， PTEN and p27kip1may play a vital role in ESCC development，metastasis and prognosis. Detection of the expression of Cyclin A， PTEN and p27kip1 is valuable in evaluating of the biological behavior and prognosis of ESCC.

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Expressions of AIB1 and epithelial-mesenchymal transition-related proteins in invasive breast cancer and their correlations

ZENG Huihui， ZHENG Rongsheng， TAO Cuiyun.

Chinese Clinical Oncology. 2015, 20 (3): 222.

Abstract ( 901 )

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Objective To investigate the expressions of AIB1 and epithelialmesenchymal transition（EMT）-related proteins in invasive breast cancer and their correlations. Methods The expressions of AIB1， E-cadherin， N-cadherin in 80 cases with invasive breast cancer， 40 cases with breast fibroadenoma tissues， 40 cases with adjacent normal breast tissues were detected by immunohistochemisty. The relationship between the expressions of AIB1， EMT-related proteins and the clinicopathological characteristics was also analyzed. Results The positive expression rate of AIB1， E-cadherin， N-cadherin was 47.5％， 36.3％， 51.3％ respectively in invasive breast cancer. AIB1 was related with lymph node metastasis and molecular typing（P＜0.05）. E-cadherin was related with histological grade， lymph node metastasis， tumor size， TNM staging（P＜0.05）. N-cadherin was related with lymph node metastasis， TNM staging（P＜0.05）. The expressions of AIB1 and E-cadherin was negatively related， but the expression of AIB1 was positively related to HER-2 and N-cadherin. The expression of E-cadherin was positively correlated with the expression of ER. Conclusion AIB1 and EMT-related proteins may be involved in the occurrence and development， and AIB1 may regulate the expression of EMT-related proteins and play an important role in the process of invasion and metastasis of invasive breast cancer.

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Expression and clinical significance of miR-29c， miR-133b and miR-1 in ovarian cancer tissue

MU Peng， ZHANG Jingru.

Chinese Clinical Oncology. 2015, 20 (3): 228.

Abstract ( 879 )

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Objective To explore the expressions of miR-29c， miR-133b and miR-1 in ovarian cancer tissue and analyze the relationship between the above indicators and clinical pathology parameters of ovarian cancer. Methods Cancer tissue from 65 patients with ovarian cancer were collected as cancer group. The real-time quantitative PCR （qRT-PCR） was used to detect the expressions of miR-29c， miR-133b and miR-1 and the clinical pathology parameters of ovarian cancer （age， clinical stage， degree of differentiation， histological type and lymph node metastasis） were collected. Meanwhile， 32 cases of normal ovarian epithelial tissues （normal group） and 39 cases of benign ovarian cyst tissue （benign group） were taken as control. The average level of three indices of normal group were chosen as boundary value， and then the patients were categorized into high-level group （＞boundary value） and low-level group （≤boundary value）. The clinical pathology parameters of patients with different levels of miR-29c， miR-133b and miR-1 were compared. The relationships among miR-29c， miR-133b and miR-1 were investigated in cancer group. Results There were higher levels of miR-29c and but lower miR-133b and miR-1 in ovarian cancer group versus other two groups with significant difference （P＜0.05）. In ovarian cancer， the levels of three miRNAs were related with degree of differentiation， and miR-29c was related with clinical stage and lymph node metastasis， and miR-133b was related with lymph node metastasis （P＜0.05）. The level of miR-29c was negatively correlated with miR-133b and miR-1 （r=-0.541, r=-0.361）， and miR-133b was positively correlated with miR-1 with statistically significance （P＜0.05）. Conclusion There were higher expression of miR-29c but lower expression of miR-133b and miR-1. The levels of the above miRNAs were related with clinical pathology parameters in ovarian cancer tissue， showing a certain value in the diagnosis of ovarian cancer.

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Expression and clinical significance of β-catenin，RIPK4 and CDK8 in mantle cell lymphoma

LI Jin， ZHONG Meizuo.

Chinese Clinical Oncology. 2015, 20 (3): 233.

Abstract ( 809 )

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Objective To detect the expressions of β-catenin， RIPK4， CDK8 and assess the relationship between the expressions of above proteins in mantle cell lymphoma（MCL） with their clinicopathological characteristics and prognosis. Methods Strept AvidinBiotin Complex immunohistochemistry was used to detect the expression of β-catenin， RIPK4 and CDK8 in 30 samples of MCL and 16 samples of lymphadenitis. All MCL patients were followed-up. The expressions of β-catenin， RIPK4 and CDK8 in MCL and lymphadentis tissues were compared. The correlation of above proteins and the relationship between the expression of them in MCL with their clinicopathological characteristics and prognosis were analyzed. Results Positive expression rates of β-catenin， RIPK4 and CDK8 in MCL tissues were 55.3％, 63.3％ and 60.0％， which were higher than those of lymphadenitis samples（P＜0.05）. There was a positive correlation among the expression of βcatenin， RIPK4 and CDK8（r=0.600，r=0.675，r=0.367，P＜0.05）. The expression of β-catenin， RIPK4 and CDK8 had no significant correlation with the gender， age， MIPI score， the level of β2-microglobulin， B symptoms and clinical stage（P＞0.05）. The median overall survival （OS） of MCL patients was 21 months. MIPI score of high risk group， the positive expression of CDK8 were independent predictors of OS. Conclusion There were high expression rate of β-catenin， RIPK4 and CDK8 proteins in MCL. There was a positive correlation among the expression of β-catenin， RIPK4 and CDK8. The positive expression of CDK8 indicated a poor prognosis of MCL.

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Manifestation of vasculogenic mimicry in osteosarcoma and its prognostic significance

REN Ke， LU Xiao， HUANG Weiqian， SHI Xin， WU Sujia， SUN Xiaoliang.

Chinese Clinical Oncology. 2015, 20 (3): 238.

Abstract ( 859 )

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Objective To investigate whether vasculogenic mimicry（VM） was present in osteosarcoma and its relevance with patients' clinicopathologic features and prognosis. Methods VM was assessed in osteosarcoma by CD34／PAS doublestaining of specimens from 66 patients. VM channels were verified to be of osteoblastic origin by staining for osteonectin and osteocalcin， and tumors were also immunohistochemically stained for focal adhesion kinase（FAK） and migration inducing gene-7（Mig-7） to determine whether these markers are associated with the occurrence of VM. The relevance of VM with the prognosis was also investigated. Results VM was observed in 15 of the 66 osteosarcoma samples（22.7％）， and the incidence of VM didn't differ with respect to patient sex， age， tumor size， tumor site， surgical type or histological response to pre-operative chemotherapy. However， Kaplan-Meier survival analysis determined that the presence of VM and the tumor necrosis rate after preoperative chemotherapy were associated with both the overall survival（P=0.011 and 0.040， respectively） and metastasis-free survival（P=0.002 and 0.045， respectively）. Furthermore， Cox proportional hazards analysis showed that the presence of VM and the histological response to preoperative chemotherapy were independent indicators for both poor overall survival（P=0.007 and 0.024， respectively） and poor metastasis-free survival（P=0.002 and 0.027， respectively）. The expression level of FAK and Mig-7 were higher in VM group than nonVM group（P=0.017 and 0.021， respectively）. Conclusion These results demonstrate the presence of VM in osteosarcoma and suggest that VM is an unfavorable prognostic factor with FAK and Mig-7 expression as a potential associated mechanism of VM formation in osteosarcoma.

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Comparative analysis of outcomes and incidences of complication in ⅠB-ⅡA cervix cancer with different treatment in Xinjiang

ADALAITI Yasheng， HUERXIDAN Niyazi，ZHANG Songan， ZHANG Lei， ZHAO Huarong.

Chinese Clinical Oncology. 2015, 20 (3): 244.

Abstract ( 877 )

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Objective To compare the outcomes and incidences of complication inⅠB-ⅡA cervix cancer with different treatment in Xinjiang. Methods In a retrospective study， the clinical and pathological data of 215 patients with ⅠB-ⅡA cervical cancer were collected as well as follow-up data. The overall 5-year survival rate was investigated and the stratification analysis of 5-year survival rate was made by clinicopathological parameters. Cox model was used to analyze the independent prognostic factors. According to treatment regimens， 215 patients were assigned into 4 groups： radical operation （n=81）， radical radiotherapy （n=65）， preoperative adjuvant therapy （n=25） and postoperative adjuvant therapy （n=44）. The prognosis and complications were analyzed among 4 groups. Results The overall 5-year survival rate was 79.3％， and varied among different FIGO stages， nations，tumor sizes， degrees of differentiation and postoperative risk factor （P＜0.05）. Cox model showed that clinical stage， tumor size， degrees of differentiation and postoperative risk factor were independent prognostic factors for cervical cancer （P＜0.05）. The 5-year survival rates were 80.9％， 82.5％， 78.8％ and 72.4％ and the incidence rates of complication were 25.9％， 18.5％， 24.0％ and 38.6％ for patients receiving radical operation， radical radiotherapy， preoperative adjuvant therapy or postoperative adjuvant therapy with no significant differences （P＞0.05）. Conclusion For patients with postoperative risk factors and preoperative large tumor volume， the corresponding postoperative adjuvant therapy can achieve similar effect as radical operation or radical radiotherapy for patients without risk factors. The individual treatment was recommended for different patients in the clinical work. In the premise of ensuring the therapeutic effect， every possible effort should be made to reduce complications of patients.

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Efficacy and safety of raltitrexed in the interventional treatment of advanced primary hepatic cancer

TANG Jie， ZHU Xiaoli， SHEN Jian， WANG Wansheng， Li Wanci.

Chinese Clinical Oncology. 2015, 20 (3): 249.

Abstract ( 950 )

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Objective To evaluate efficacy and safety of advanced primary hepatic carcinoma（PHC） by transcatheter arterial chemoembolization（TACE） with raltitrexed treatment. Methods All 95 patients with advanced PHC confirmed by pathology or clinical diagnosis were enrolled from January 2012 to December 2013， dividing into experimental group （n=37） and control group （n=58）. Experimental group： raltitrexed （3 mg／m2） plus oxaliplatin （100 mg／m2）， pirarubicin （40 mg／m2） in TACE treatment， while control group： FUDR （1.0 g） combined with oxaliplatin （100 mg／m2）， pirarubicin （40 mg／m2）. Repeated treatment was given every 4-6 weeks and efficacy was evaluated after four weeks in accordance with the modified RECIST criteria （mRECISIT）， until efficacy evaluation for complete remission （CR）， liver function Childpugh C level or intolerable complications were occured. The response rate（RR）， disease control rate（DCR）， decreasing rate of alphafetoprotein （AFP）， progression free survival （PFS） and the incidence of adverse reactions were assessed， and the results were compared between the two groups. Results In experimental group and control group， the RR was 351％ and 327％（P＞005）， AFP decreasing rate was 40.5％ and 31.0％（P＞0.05）， the median PFS was 7 and 5 months（P＞0.05）， DCR were 86.5％， 67.2％（P=0.035），respectively. The main adverse events in both groups were nausea， vomiting， bone marrow suppression， fever and transaminase increase， the difference was not statistically significant （P＞0.05）. Conclusion Preliminary study has shown that the short-term curative effect of PHC by TACE with raltitrexed treatment is exact and the toxicity can be tolerated. But it still needs a large randomized controlled study to confirm the results.

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Clinical observation of the intensity modulated radiotherapy in cervical stump cancer

XU Hanzi， LU Emei， SUN Zhihua， WU Qiang， ZHANG Xiuming， GONG Zhen.

Chinese Clinical Oncology. 2015, 20 (3): 253.

Abstract ( 809 )

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Objective To evaluate the efficacy and side effects of the intensity modulated radiotherapy in cervical stump cancer. Methods A retrospective study included 41 patients from January 1994 to December 2013 with cervical stump cancer was performed. Before 2012， 18 cases as control group were received conventional external beam radiotherapy and brachytherapy. Since 2012，23 cases as experimental group were treated with intensity modulated radiotherapy（IMRT） and brachytherapy. The efficacy and complications of two groups were evaluated. Results In the control group，there was 8 cases CR， 6 cases PR， and the response rate was 77.8％， and the decreasing rate of SCC was（61.3±20.4）％. In the experimental group， there was 12 cases CR， 8cases PR， and the response rate was 87.0％， and the decreasing rate of SCC was（75.9±4.3）％. There was 17 patients suffered toxicities including 13 grade 1-2 acute rectum reaction，4 grade 1 bladder reaction the control group， while in the experimental group， only 6 patients suffered grade 1 acute rectum reaction. Conclusion The IMRT and braehytherapy for the patients with cervical stump cancer have better effects and less complications.

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Correlation between ultrasonic TN staging and postoperative pathology in rectal cancer after neoadjuvant radiochemotherapy

WANG Ming， LI Xingde， ZHU Zhongcheng， ZHU Shuchai.

Chinese Clinical Oncology. 2015, 20 (3): 257.

Abstract ( 784 )

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Objective To evaluate the accuracy of endorectal ultrasound （ERUS） in the TN staging of rectal cancer after neoadjuvant chemoradiation in comparison with postoperative pathology. Methods Forty patients with rectal cancer were chosen in our hospital from January 2013 to December 2013. All patients received dimensional conformal radiotherapy or intensity modulated radiotherapy （DT50Gy／25 fractions）. From the first day of radiotherapy， S-1 capsules were administered orally for two periods which including two weeks administration and one week drug withdrawal. Operations were carried out in six to eight weeks after chemoradiation. Patients were given ERUS before operation and postoperative pathology. Stages of patients before and after operation were compared. Results The diagnosis accuracy rates of ERUS for T1， T2， T3 and T4 stage were 77.8％， 76.9％， 83.3％ and 83.3％， respectively. The total diagnosis accuracy rate of T stage was 80.0％. The diagnosis accuracy rates of endosonography for N0 and N+ stage were 88.4％ and 62.5％， respectively. The total diagnosis accuracy rate of N stage was 80.0％. The total diagnosis accuracy rate of TN was 72.5％. Conclusion ERUS has good accuracy in T stage and N stage diagnosis. It is one of the most common used detective methods in preoperative diagnosis of rectal cancer.

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TOB1 gene of antiproliferative family and cancer

HE Hongjie， YU Jingcui.

Chinese Clinical Oncology. 2015, 20 (3): 262.

Abstract ( 816 )

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Transducer of ErbB-2.1 （TOB1） encodes Tob1 protein which is a member of the BTG／TOB antiproliferative protein family. It is involved in regulating cell cycle progression through Ras／MAPK pathway and forming complex with CCR4Caf1 to inhibit cell proliferation. TOB1 as a tumor suppressor gene is closely related with tumor occurrence， development and metastasis. The decreased expression of Tob1 occurred in the majority of tumor. Its functional inactivation is associated with phosphorylation of accumulation and subcellular distribution， and the expression of TOB1 is associated with ionization sensitivity.

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Long non-coding RNAs： A new frontier in the study of nonsmall cell lung cancer

ZHOU Peng， ZHANG Hanjie， LIU Xianghua.

Chinese Clinical Oncology. 2015, 20 (3): 266.

Abstract ( 717 )

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Non-small cell lung cancer （NSCLC） including adenocarcinoma and squamous cell carcinoma， is the predominant form of lung cancer， and accounts for the majority of cancer deaths worldwide. Despite recent advances in clinical and experimental oncology， the prognosis of lung cancer is still unfavorable， with a 5-year overall survival rate of approximately 17％. Thus， a detailed understanding of the mechanisms underlying NSCLC development and progression is essential for improving the diagnosis， prevention and treatment of this disease. Recently， accumulating evidence has shown that long noncoding RNA （LncRNA） may be involved in NSCLC pathogenesis， playing an important role in invasion and metastasis， proliferation， apoptosis and drug resistance of NSCLC cell. Here， we summarize the uptodate research progress on LncRNAs in NSCLC to provide new insights into the genetic diagnosis and treatment of this deadly disease.

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BRAF mutation and BRAF-targeted therapy： recent advances in non-small cell lung cancer

ZHANG Jing， FAN Min.

Chinese Clinical Oncology. 2015, 20 (3): 271.

Abstract ( 829 )

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BRAF mutation is one of driver mutations in non-small cell lung cancer（NSCLC）. BRAF mutation rate is about 0.5％～4.9％， and more than half of BRAF mutation is V600E. BRAF mutation occurs more commonly in female patients with adenocarcinoma， and the mutation rate is much lower in Asian people. The clinical significance of coexistence of BRAF mutation and other mutations， such as EGFR and K-Ras mutations was yet unknown. Given the huge global burden of lung cancer， progress of BRAF mutation studies hold significant importance， despite its low mutation rate overall. Currently， several BRAF inhibitors are being studied in clinical trials for patients with NSCLC. This review is intended to outline the recent advances of BRAF mutation and its targeted therapy in NSCLC.

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Application status and progression of thalidomide in treatment of solid tumors

WANG Nianfei， CHEN Zhendong.

Chinese Clinical Oncology. 2015, 20 (3): 276.

Abstract ( 928 )

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Thalidomide originally was used for the treatment of vomiting of pregnancy， which was once forbidden in the clinical for teratogenic events. Basic researches have confirmed that thalidomide has the effect of antiangiogenesis， immune adjustment and anti-inflammatory. Therefore， it is postulated that it has antitumor potential， as evident by the treatment of multiple myeloma. The treatment of solid tumors by thalidomide is still in the exploration stage. The existing literatures are focusing on case reports and phase Ⅰ and Ⅱ clinical study with inconsistent curative effect， needing to be further confirmed by largesample randomized controlled clinical trials. In addition， thalidomide may have a certain value to relieve the gastrointestinal reaction caused by chemotherapy and cancer anorexia cachexia syndrome.

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标准刊号：	ISSN 1009-0460
	CN 32-1577/R